Immobilization of trypsin on alginic acid-poly (glycidyl methacrylate) graft copolymer
نویسندگان
چکیده
منابع مشابه
Immobilization of gelatin onto poly(glycidyl methacrylate)-grafted polycaprolactone substrates for improved cell-material interactions.
To enhance the cytocompatibility of polycaprolactone (PCL), cell-adhesive gelatin is covalently immobilized onto the PCL film surface via two surface-modified approaches: a conventional chemical immobilization process and a surface-initiated atom transfer radical polymerization (ATRP) process. Kinetics studies reveal that the polymer chain growth from the PCL film using the ATRP process is form...
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In this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly(ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (PDMS) and cyclic olefin copolymer (COC...
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Hydrogels based on 2-hydroxyethyl methacrylate/dimethyl-aminoethyl methacrylate copolymers were prepared by gamma radiation-inducedco-polymerization at low temperature (−78◦C). The swelling behavior of hydrogels was studied by immersion of the polymer discs in buffered solutions at pH from 2 to 10. The hydration process was followed gravimetrically by measuring the water uptake of the discs as ...
متن کاملCisplatin-incorporated nanoparticles of poly(acrylic acid-co-methyl methacrylate) copolymer
BACKGROUND Although cisplatin is extensively used in the clinical field, its intrinsic toxicity limits its clinical use. We investigated nanoparticle formations of poly(acrylic acid-co-methyl methacrylate) (PAA-MMA) incorporating cisplatin and their antitumor activity in vitro and in vivo. METHODS Cisplatin-incorporated nanoparticles were prepared through the ion-complex formation between acr...
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Well-shaped spherical magnetic microspheres were prepared using a synthetic polymer of poly-(acrylonitrile-co-acrylamide). Their size varied from 100 up to 1000 μm proportionally to the flow rate of the polymer solution and their density was from 1030 to 1045 kg.m, depending on the magnetite content. Trypsin (EC 3.4.21.4) was chosen as model enzyme and immobilized on the acyl azide derivatives ...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1986
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260280419